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1 of 25
Q.1 Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
2 of 25
Q.2 In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
3 of 25
Q.3 The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
4 of 25
Q.4 Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
5 of 25
Q.5 The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
6 of 25
Q.6 While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
7 of 25
Q.7 Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
8 of 25
Q.8 Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
9 of 25
Q.9 Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
10 of 25
Q.10 Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
11 of 25
Q.11 The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
12 of 25
Q.12 In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
13 of 25
Q.13 DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
14 of 25
Q.14 Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
15 of 25
Q.15 Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
16 of 25
Q.16 Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
17 of 25
Q.17 Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
18 of 25
Q.18 Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
19 of 25
Q.19 Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
20 of 25
Q.20 Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
21 of 25
Q.21 DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
22 of 25
Q.22 Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
23 of 25
Q.23 Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
24 of 25
Q.24 Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
25 of 25
Q.25 Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes