This practice test consists of 25 important questions from the Biology Chapter - Biotechnology: Principles And Processes (Class 12). these questions and practice test is helpful for NEET Ug and other competitive entrance exams.
To Check your Progress and get points for your performance
Login & Start the Test
OR
Start the Test Without Login
OR
Scroll Down to See Questions
Q.1.
Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
Answer:
(a) introducing rDNA into plant cells
Explanation: Biolistics (gene gun) is suitable for introducing rDNA into plant cells
Explanation: Biolistics (gene gun) is suitable for introducing rDNA into plant cells
Q.2.
In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
Answer:
(b) bacterial DNA
Explanation: In genetic engineering experiments, restriction enzymes are used for bacterial DNA
Explanation: In genetic engineering experiments, restriction enzymes are used for bacterial DNA
Q.3.
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Answer:
(b) gel electrophoresis
Explanation: The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
Explanation: The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
Q.4.
Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
Answer:
()
Explanation: Plasmids in bacterial cells are extra-chromosomal DNA, which can . self-replicate
Explanation: Plasmids in bacterial cells are extra-chromosomal DNA, which can . self-replicate
Q.5.
The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
Answer:
(a) Thermus aquaticus
Explanation: The DNA polymerase enzyme used in PCR is obtained from Thermus aquaticus
Explanation: The DNA polymerase enzyme used in PCR is obtained from Thermus aquaticus
Q.6.
While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
Answer:
(a) Lysozyme
Explanation: Lysozyme is not used
Explanation: Lysozyme is not used
Q.7.
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Answer:
(c) uptake of DNA through transient pores in the bacterial cell wall
Explanation: uptake of DNA through transient pores in the bacterial cell wall
Explanation: uptake of DNA through transient pores in the bacterial cell wall
Q.8.
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
Answer:
(c) Extension of primer end on the template DNA
Q.9.
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
Answer:
(d) Ethidium bromide stained DNA can be seen under exposure to UV light
Explanation: Ethidium bromide stained DNA can be seen under exposure to UV light is the correct answer
Explanation: Ethidium bromide stained DNA can be seen under exposure to UV light is the correct answer
Q.10.
Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
Answer:
(c) prevention of the multiplication of bacteriophage in bacteria
Explanation: Restriction’ in Restriction enzyme refers to prevention of the multiplication of bacteriophage in bacteria
Explanation: Restriction’ in Restriction enzyme refers to prevention of the multiplication of bacteriophage in bacteria
Q.11.
The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
Answer:
(b) formation of hydrogen bonds between sticky ends of DNA fragments
Q.12.
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
Answer:
(c) transformants only and not the non-transformants can survive
Explanation: transformants only and not the non-transformants can survive
Explanation: transformants only and not the non-transformants can survive
Q.13.
DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
Answer:
(c) Intron
Explanation: DNA element with ability to change its position is called Intron
Explanation: DNA element with ability to change its position is called Intron
Q.14.
Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
Answer:
(c) Bacteriophage
Explanation: Viral genome incroporatcd into host DNA is called Bacteriophage
Explanation: Viral genome incroporatcd into host DNA is called Bacteriophage
Q.15.
Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
Answer:
(d) Barley
Explanation: Triticale, first man made cereal crop, has been obtained by crossing wheat with Barley
Explanation: Triticale, first man made cereal crop, has been obtained by crossing wheat with Barley
Q.16.
Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
Answer:
(d) High vitamin A content
Explanation: It will help in High vitamin A content.
Explanation: It will help in High vitamin A content.
Q.17.
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
Answer:
(d) Bacterial cells
Explanation: Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from Bacterial cells
Explanation: Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from Bacterial cells
Q.18.
Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
Answer:
(b) Both strands of DNA
Explanation: Restriction endonuclease cuts both strands of DNA
Explanation: Restriction endonuclease cuts both strands of DNA
Q.19.
Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
Answer:
(d) Extra chromosomal DNA in bacterial cell
Explanation: Plasmid is a extra chromosomal DNA in bacterial cell
Explanation: Plasmid is a extra chromosomal DNA in bacterial cell
Q.20.
Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
Answer:
(a) Excherichia coli and Agrobacterium tumefaciens
Explanation: Excherichia coli and Agrobacterium tumefaciens are two microbes found to be very useful in Genetic Engineering
Explanation: Excherichia coli and Agrobacterium tumefaciens are two microbes found to be very useful in Genetic Engineering
Q.21.
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Answer:
(a) W.B.C
Q.22.
Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
Answer:
(a) Smith and Nathane
Explanation: Smith and Nathane discovered Restricted endonucleas enzymes
Explanation: Smith and Nathane discovered Restricted endonucleas enzymes
Q.23.
Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
Answer:
(a) Escherichiya Coli and Agrobacterium tumefaciens
Explanation: Two microbes found to be very useful in genetic, engineering are Escherichiya Coli and Agrobacterium tumefaciens
Explanation: Two microbes found to be very useful in genetic, engineering are Escherichiya Coli and Agrobacterium tumefaciens
Q.24.
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Answer:
(a) Introducing of foreign fenes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Q.25.
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Answer:
(a) Introducing of foreign fenes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Explanation: Transgenic plants are develop by Introducing of foreign genes
SIMILAR FOR YOU
Videos from Youtube 〉
Posted in class 12th biology class 12th biology chapterwise class 12th biology for neet class 12th biology questions class 12th biology viva questions neet 12th 12th class biology 12th class biology for neet free neet preparation neet chapter wise mcqs neet class 12th neet exam 2022 neet important biology questions neet important questions neet practice neet questions neet ug practice questions neet ug 2024 neet ug exam 2024 neet ug practice biotechnology biotechnology principles and processes biotechnology principles biotechnology practice questions biotechnology mcqs biotechnology mcqs for neet biotechnology revision questions biotechnology viva questions biotechnology class 12th biotechnology class xii
1 likes 2715 views
Share