This practice test consists of 25 important questions from the Biology Chapter - Biotechnology: Principles And Processes (Class 12). these questions and practice test is helpful for NEET Ug and other competitive entrance exams.
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Q.1.
Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
Answer:
(a) introducing rDNA into plant cells
Explanation: Biolistics (gene gun) is suitable for introducing rDNA into plant cells
Explanation: Biolistics (gene gun) is suitable for introducing rDNA into plant cells
Q.2.
In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
Answer:
(b) bacterial DNA
Explanation: In genetic engineering experiments, restriction enzymes are used for bacterial DNA
Explanation: In genetic engineering experiments, restriction enzymes are used for bacterial DNA
Q.3.
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Answer:
(b) gel electrophoresis
Explanation: The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
Explanation: The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
Q.4.
Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
Answer:
()
Explanation: Plasmids in bacterial cells are extra-chromosomal DNA, which can . self-replicate
Explanation: Plasmids in bacterial cells are extra-chromosomal DNA, which can . self-replicate
Q.5.
The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
Answer:
(a) Thermus aquaticus
Explanation: The DNA polymerase enzyme used in PCR is obtained from Thermus aquaticus
Explanation: The DNA polymerase enzyme used in PCR is obtained from Thermus aquaticus
Q.6.
While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
Answer:
(a) Lysozyme
Explanation: Lysozyme is not used
Explanation: Lysozyme is not used
Q.7.
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Answer:
(c) uptake of DNA through transient pores in the bacterial cell wall
Explanation: uptake of DNA through transient pores in the bacterial cell wall
Explanation: uptake of DNA through transient pores in the bacterial cell wall
Q.8.
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
Answer:
(c) Extension of primer end on the template DNA
Q.9.
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
Answer:
(d) Ethidium bromide stained DNA can be seen under exposure to UV light
Explanation: Ethidium bromide stained DNA can be seen under exposure to UV light is the correct answer
Explanation: Ethidium bromide stained DNA can be seen under exposure to UV light is the correct answer
Q.10.
Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
Answer:
(c) prevention of the multiplication of bacteriophage in bacteria
Explanation: Restriction’ in Restriction enzyme refers to prevention of the multiplication of bacteriophage in bacteria
Explanation: Restriction’ in Restriction enzyme refers to prevention of the multiplication of bacteriophage in bacteria
Q.11.
The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
Answer:
(b) formation of hydrogen bonds between sticky ends of DNA fragments
Q.12.
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
Answer:
(c) transformants only and not the non-transformants can survive
Explanation: transformants only and not the non-transformants can survive
Explanation: transformants only and not the non-transformants can survive
Q.13.
DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
Answer:
(c) Intron
Explanation: DNA element with ability to change its position is called Intron
Explanation: DNA element with ability to change its position is called Intron
Q.14.
Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
Answer:
(c) Bacteriophage
Explanation: Viral genome incroporatcd into host DNA is called Bacteriophage
Explanation: Viral genome incroporatcd into host DNA is called Bacteriophage
Q.15.
Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
Answer:
(d) Barley
Explanation: Triticale, first man made cereal crop, has been obtained by crossing wheat with Barley
Explanation: Triticale, first man made cereal crop, has been obtained by crossing wheat with Barley
Q.16.
Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
Answer:
(d) High vitamin A content
Explanation: It will help in High vitamin A content.
Explanation: It will help in High vitamin A content.
Q.17.
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
Answer:
(d) Bacterial cells
Explanation: Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from Bacterial cells
Explanation: Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from Bacterial cells
Q.18.
Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
Answer:
(b) Both strands of DNA
Explanation: Restriction endonuclease cuts both strands of DNA
Explanation: Restriction endonuclease cuts both strands of DNA
Q.19.
Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
Answer:
(d) Extra chromosomal DNA in bacterial cell
Explanation: Plasmid is a extra chromosomal DNA in bacterial cell
Explanation: Plasmid is a extra chromosomal DNA in bacterial cell
Q.20.
Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
Answer:
(a) Excherichia coli and Agrobacterium tumefaciens
Explanation: Excherichia coli and Agrobacterium tumefaciens are two microbes found to be very useful in Genetic Engineering
Explanation: Excherichia coli and Agrobacterium tumefaciens are two microbes found to be very useful in Genetic Engineering
Q.21.
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Answer:
(a) W.B.C
Q.22.
Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
Answer:
(a) Smith and Nathane
Explanation: Smith and Nathane discovered Restricted endonucleas enzymes
Explanation: Smith and Nathane discovered Restricted endonucleas enzymes
Q.23.
Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
Answer:
(a) Escherichiya Coli and Agrobacterium tumefaciens
Explanation: Two microbes found to be very useful in genetic, engineering are Escherichiya Coli and Agrobacterium tumefaciens
Explanation: Two microbes found to be very useful in genetic, engineering are Escherichiya Coli and Agrobacterium tumefaciens
Q.24.
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Answer:
(a) Introducing of foreign fenes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Q.25.
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Answer:
(a) Introducing of foreign fenes
Explanation: Transgenic plants are develop by Introducing of foreign genes
Explanation: Transgenic plants are develop by Introducing of foreign genes
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