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RRB Laboratory Assistant Grade II (MLT) CBT Mock Test 1

RRB Laboratory Assistant Grade II (MLT) CBT Mock Test 1

Rohan Joshi October 21, 2026 92 views 100 Questions
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Q1. What is the standard concentration and volume of neutral buffered formalin used as a universal tissue fixative in histopathology?
Q2. Under Biomedical Waste Management Rules 2016, into which color-coded container must contaminated solid plastic labware (e.g. pipette tips, plastic petri dishes, vacutainers) be discarded?
Q3. According to the Westgard multirule system for clinical laboratory quality control, a '1_3s' rule violation indicates:
Q4. Which optical part of a brightfield compound microscope concentrates and directs light onto the specimen slide?
Q5. What is the primary function of a Class II biological safety cabinet (BSC), commonly used for aerosol-generating procedures in a BSL-2 diagnostic laboratory?
Q6. Which anticoagulant acts by chelating ionized calcium (Ca2+) and is universally preferred for routine complete blood counts (CBC)?
Q7. What is the recommended ratio of 3.2% (0.109 M) buffered Sodium Citrate anticoagulant to venous blood for coagulation profile testing (PT/INR and aPTT)?
Q8. Sodium Fluoride is incorporated alongside potassium oxalate into grey-top blood collection tubes specifically to:
Q9. Which chemical agent is commonly added to 10% formalin to formulate Bouin's fixative, excellent for testicular and endocrine biopsy preservation?
Q10. In clinical spectrophotometry, which wavelength region represents the Ultraviolet (UV) spectrum used for measuring enzymatic NAD/NADH reactions?
Q11. In routine paraffin tissue processing, which reagent is universally employed as a 'Clearing Agent' between dehydration and wax infiltration?
Q12. What is the recommended melting temperature range for histological embedding grade Paraffin Wax?
Q13. In Hematoxylin and Eosin (H&E) staining, what is the chemical role of 'Alum' (e.g. Ammonium or Potassium aluminum sulfate) in Harris or Mayer's hematoxylin?
Q14. In a well-stained H&E histological section, which color do cell nuclei and cell cytoplasm display respectively?
Q15. Which special histochemical stain is diagnostic for demonstrating Glycogen, neutral mucins, and fungal cell walls in tissue sections?
Q16. Perls' Prussian Blue reaction in histopathology is utilized for the specific demonstration of:
Q17. Which microtome knife angle refers to the angle formed between the cutting facet of the knife and the surface of the tissue block?
Q18. What is the recommended fixative of choice for cervical smear (Pap smear) cytology slides?
Q19. In Papanicolaou (Pap) staining of vaginal smears, which dye specifically stains the cytoplasm of superficial mature keratinized squamous cells orange?
Q20. Which microtome is most suitable for cutting ultra-thin sections (50 to 100 nm) for Transmission Electron Microscopy (TEM)?
Q21. Which enzymatic method is commonly regarded as the reference method for quantitative blood glucose measurement on clinical chemistry analyzers?
Q22. Jaffe's reaction is the classic chemical colorimetric method employed for the laboratory estimation of:
Q23. Which serum enzyme elevation is most specific for hepatocellular injury (acute viral hepatitis, toxin-induced liver injury)?
Q24. What is the normal reference range for Fasting Blood Glucose (FBG) in a healthy non-diabetic adult according to ADA guidelines?
Q25. Serum alkaline phosphatase (ALP) is often markedly elevated in which liver-related pathological condition?
Q26. Glycated Hemoglobin (HbA1c) measurement reflects the mean circulating blood glucose level over which preceding timeframe?
Q27. Which lipoprotein is clinically termed 'Good Cholesterol' due to its role in reverse cholesterol transport from peripheral tissues to the liver?
Q28. The Uric Acid test is of principal diagnostic value in the clinical evaluation and monitoring of which metabolic disease?
Q29. In the biuret method for total serum protein estimation, what reagent reacts with peptide bonds in an alkaline medium to produce a violet-colored chelate?
Q30. Which dye-binding method is commonly used for colorimetric estimation of serum albumin?
Q31. What is the normal reference range for Serum Potassium (K+) in healthy adults?
Q32. Which cardiac enzyme marker was traditionally used to support the diagnosis of acute myocardial infarction, although cardiac troponins are now preferred?
Q33. Elevated serum lipase, especially with elevated amylase and compatible symptoms, supports the diagnosis of which acute abdominal condition?
Q34. Which hormone estimation is used as the initial, most sensitive first-line screening investigation for primary thyroid dysfunction?
Q35. An arterial blood gas shows pH 7.25, PaCO₂ 28 mmHg, and HCO₃⁻ 14 mEq/L. What is the primary acid–base disorder?
Q36. In the Gram staining procedure, what is the sequence and role of the four classic reagents used?
Q37. Which bacterial structure is primarily responsible for resistance to physical heat, desiccation, and chemical disinfectants in Bacillus and Clostridium species?
Q38. MacConkey Agar is classified as which type of culture medium?
Q39. In the conventional Ziehl–Neelsen acid-fast staining method, which combination is used as the primary stain and decolorizer?
Q40. Which egg-based solid culture medium is commonly used for cultivating Mycobacterium tuberculosis?
Q41. In clinical bacteriology, which rapid enzymatic test differentiates pathogenic Staphylococcus aureus (positive) from coagulase-negative Staphylococci like S. epidermidis (negative)?
Q42. The standard disk diffusion method for Antimicrobial Susceptibility Testing (AST) recommended by CLSI is known as:
Q43. A 10% Potassium Hydroxide (KOH) wet mount of skin scrapings, hair, or nail clippings is performed in the laboratory to detect:
Q44. India Ink negative staining of cerebrospinal fluid (CSF) is diagnostic for demonstrating the thick gelatinous capsule of which fungal pathogen?
Q45. Which hepatitis B serological marker is generally the earliest routinely detectable marker of a new HBV infection in blood?
Q46. Which of the following describes the principle of the standard Sandwich ELISA (Enzyme-Linked Immunosorbent Assay)?
Q47. Which selective agar medium with high pH (8.6) and sucrose is designed for the isolation of Vibrio cholerae from cholera stool specimens?
Q48. The Widal test used in the presumptive serodiagnosis of Enteric (Typhoid) Fever detects circulating serum agglutinins against which antigens of Salmonella enterica?
Q49. Which morphological feature definitively distinguishes the quadrinucleate cyst of pathogenic Entamoeba histolytica from the commensal Entamoeba coli?
Q50. Which intestinal flagellate protozoan displays a characteristic 'falling-leaf motility' in fresh warm saline stool wet mounts?
Q51. Bile-stained, barrel-shaped fertilized eggs with prominent bipolar transparent mucus plugs are characteristic of which nematode parasite?
Q52. Which standard collection method is used to detect Enterobius vermicularis (pinworm) eggs on perianal skin, especially in children?
Q53. In peripheral blood smear examination for malaria, crescent- or banana-shaped gametocytes are uniquely diagnostic of which Plasmodium species?
Q54. Compared with a thin blood smear, what is the main advantage of a thick blood smear for malaria screening?
Q55. Wuchereria bancrofti microfilariae in nocturnal lymphatic filariasis exhibit nocturnal periodicity, meaning optimal blood collection must be performed between:
Q56. What is the principle of the Cyanmethemoglobin (HiCN) reference method for Hemoglobin determination using Drabkin's solution?
Q57. In the standard Improved Neubauer Hemocytometer grid, what is the depth of the counting chamber between the glass cover slip and counting platform?
Q58. Which red blood cell index represents the average volume of individual red blood cells, expressed in femtoliters (fL)?
Q59. Supravital staining with New Methylene Blue or Brilliant Cresyl Blue is mandatory for identifying which immature erythrocyte containing residual RNA networks?
Q60. In the standard Westergren ESR method, what is the graduated measurement scale of the Westergren tube?
Q61. Which Romanowsky stain uses methanol as both fixative and solvent and is commonly used for peripheral blood smears?
Q62. Prothrombin time (PT/INR) primarily evaluates which coagulation pathways?
Q63. Microcytic, hypochromic red blood cells with marked anisopoikilocytosis and pencil cells on peripheral blood smear are classic morphological hallmarks of:
Q64. In blood grouping, 'Reverse (Serum) Grouping' is performed to confirm forward grouping by testing the patient's serum against known:
Q65. What is the primary clinical application of the Direct Antiglobulin Test (DAT / Direct Coombs Test)?
Q66. What is the officially mandated storage temperature range for Packed Red Blood Cells (PRBC) preserved with CPDA-1 solution?
Q67. Fresh Frozen Plasma (FFP) must be separated within 8 hours of phlebotomy and stored at what temperature to preserve labile Coagulation Factors V and VIII?
Q68. In routine urinalysis, which chemical reagent strip test or sulfosalicylic acid (SSA) precipitation test is utilized to detect abnormal levels of:
Q69. Which urinary cast most strongly suggests bleeding originating in the glomeruli?
Q70. A yellowish or orange CSF supernatant after centrifugation is termed what, and may be associated with which condition?
Q71. What is the SI unit of electric potential difference (voltage)? /विद्युत विभवांतर (वोल्टेज) का SI मात्रक क्या है?
Q72. Which of the following mirrors is commonly used as a rear-view mirror in motor vehicles? /वाहनों में पीछे का दृश्य देखने के लिए (रियर-व्यू मिरर) किस दर्पण का उपयोग किया जाता है?
Q73. What is the chemical formula of Plaster of Paris? /प्लास्टर ऑफ पेरिस का रासायनिक सूत्र क्या है?
Q74. Which metal is liquid at standard room temperature (25°C)? /कौन सी धातु सामान्य कमरे के तापमान (25°C) पर द्रव अवस्था में पाई जाती है?
Q75. What is the pH of pure water at 25°C under neutral conditions? /25°C पर शुद्ध आसुत जल (pure water) का pH मान कितना होता है?
Q76. Which cell organelle is known as the 'Powerhouse of the Cell' due to ATP generation? /ATP निर्माण के कारण कोशिका का 'पावरहाउस' (ऊर्जा घर) किसे कहा जाता है?
Q77. Which blood group is known as the 'Universal Donor' in the ABO-Rh blood grouping system? /ABO-Rh रक्त समूह प्रणाली में किस रक्त समूह को 'सार्वभौमिक दाता' (Universal Donor) कहा जाता है?
Q78. Which vitamin deficiency is the direct cause of Rickets in growing children? /बढ़ते बच्चों में रिकेट्स (सूखा रोग) किस विटामिन की कमी के कारण होता है?
Q79. What is the speed of light in vacuum? /निर्वात (vacuum) में प्रकाश की चाल कितनी होती है?
Q80. The process of conversion of atmospheric nitrogen into ammonia by soil bacteria is termed: /मृदा जीवाणुओं द्वारा वायुमंडलीय नाइट्रोजन को अमोनिया में परिवर्तित करने की प्रक्रिया क्या कहलाती है?
Q81. If a shopkeeper sells an article for Rs. 460 at a gain of 15%, what was the cost price of the article? /यदि एक दुकानदार किसी वस्तु को 15% लाभ पर 460 रुपये में बेचता है, तो वस्तु का क्रय मूल्य क्या था?
Q82. What is the simple interest on a principal of Rs. 5,000 at an annual interest rate of 8% for 3 years? /5,000 रुपये के मूलधन पर 8% वार्षिक ब्याज दर से 3 वर्ष का साधारण ब्याज कितना होगा?
Q83. A can complete a piece of work in 12 days and B can complete the same work in 24 days. Working together, in how many days will they finish the work? /A किसी कार्य को 12 दिनों में पूरा कर सकता है और B उसी कार्य को 24 दिनों में पूरा कर सकता है। दोनों मिलकर उस कार्य को कितने दिनों में समाप्त करेंगे?
Q84. A train traveling at 72 km/h crosses a 180-meter long platform in 18 seconds. What is the length of the train? /72 किमी/घंटा की गति से चल रही एक ट्रेन 180 मीटर लंबे प्लेटफॉर्म को 18 सेकंड में पार करती है। ट्रेन की लंबाई क्या है?
Q85. Find the next number in the series: 7, 14, 28, 56, 112, ? /दी गई श्रृंखला में अगला पद ज्ञात कीजिए: 7, 14, 28, 56, 112, ?
Q86. In a certain code language, a word is reversed and then each letter is shifted forward by one place in the alphabet. If 'MEDICINE' is coded using this rule, how will 'HOSPITAL' be coded? /एक निश्चित कूट भाषा में किसी शब्द को पहले उल्टा लिखा जाता है और फिर हर अक्षर को अंग्रेज़ी वर्णमाला में एक स्थान आगे बढ़ाया जाता है। इस नियम से 'MEDICINE' को कूटबद्ध करने पर 'HOSPITAL' का कोड क्या होगा?
Q87. Pointing to a photograph, a woman said, 'He is the only son of my father's father.' How is the man in the photograph related to the woman? /एक तस्वीर की ओर इशारा करते हुए एक महिला ने कहा, 'वह मेरे पिता के पिता का एकमात्र पुत्र है।' तस्वीर वाला व्यक्ति उस महिला से किस प्रकार संबंधित है?
Q88. A person walks 5 km North, turns right and walks 4 km, then turns right again and walks 5 km. How far and in which direction is he from his starting point? /एक व्यक्ति 5 किमी उत्तर की ओर चलता है, दाएं मुड़कर 4 किमी चलता है, फिर दोबारा दाएं मुड़कर 5 किमी चलता है। वह प्रारंभिक बिंदु से कितनी दूरी और किस दिशा में है?
Q89. Select the related word from the given alternatives: Heart : Cardiology :: Kidney : ? /दिए गए विकल्पों में से संबंधित शब्द चुनिए: हृदय : कार्डियोलॉजी :: वृक्क (किडनी) : ?
Q90. The ratio of two numbers is 3 : 5 and their Least Common Multiple (LCM) is 75. What is the sum of the two numbers? /दो संख्याओं का अनुपात 3 : 5 है और उनका लघुत्तम समापवर्त्य (LCM) 75 है। दोनों संख्याओं का योग क्या है?
Q91. Under the Ayushman Bharat PM-JAY scheme, what is the annual health insurance coverage provided per eligible family? /आयुष्मान भारत PM-JAY योजना के तहत प्रति पात्र परिवार को प्रति वर्ष कितना स्वास्थ्य बीमा कवर प्रदान किया जाता है?
Q92. Which Article of the Constitution of India provides for the 'Right to Protection of Life and Personal Liberty'? /भारतीय संविधान का कौन सा अनुच्छेद 'प्राण और दैहिक स्वतंत्रता के संरक्षण' (जीवन का अधिकार) का प्रावधान करता है?
Q93. Which river is known as the 'Dakshin Ganga' (Ganga of the South) owing to its size and length? /लंबाई और विशालता के कारण किस नदी को 'दक्षिण गंगा' कहा जाता है?
Q94. Who presided over the historic 1929 Lahore Session of the Indian National Congress where 'Purna Swaraj' was declared? /भारतीय राष्ट्रीय कांग्रेस के ऐतिहासिक 1929 के लाहौर अधिवेशन की अध्यक्षता किसने की थी जिसमें 'पूर्ण स्वराज' का संकल्प पारित किया गया था?
Q95. What is the primary function of the Monetary Policy Committee (MPC) constituted by the Reserve Bank of India? /भारतीय रिजर्व बैंक द्वारा गठित मौद्रिक नीति समिति (MPC) का मुख्य कार्य क्या है?
Q96. In which Indian state is the Kaziranga National Park, famous for the one-horned rhinoceros, situated? /एक सींग वाले गैंडे के लिए प्रसिद्ध काजीरंगा राष्ट्रीय उद्यान किस भारतीय राज्य में स्थित है?
Q97. Which Constitutional Amendment Act reduced the voting age in India from 21 years to 18 years? /किस संविधान संशोधन अधिनियम द्वारा भारत में मतदान की न्यूनतम आयु 21 वर्ष से घटाकर 18 वर्ष की गई थी?
Q98. Under the Pradhan Mantri Bhartiya Janaushadhi Pariyojana (PMBJP), what is the primary objective of Janaushadhi Kendras? /प्रधानमंत्री भारतीय जनऔषधि परियोजना (PMBJP) के तहत जनऔषधि केंद्रों का मुख्य उद्देश्य क्या है?
Q99. Who is known as the 'Father of the Indian Constitution'? /'भारतीय संविधान के जनक' (Father of the Indian Constitution) के रूप में किसे जाना जाता है?
Q100. Which international day is observed globally on April 7 every year to mark the founding of the World Health Organization (WHO)? /विश्व स्वास्थ्य संगठन (WHO) की स्थापना के उपलक्ष्य में प्रत्येक वर्ष 7 अप्रैल को कौन सा दिवस मनाया जाता है?

RRB Laboratory Assistant Grade II (MLT)

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Free RRB Laboratory Assistant Grade II Mock Test 2026. Practice 100 questions covering Clinical Biochemistry, Haematology, Microbiology, and General Science as per official CEN 05/2026 pattern on Solotutes.

This test includes
Technical Core (Q1–Q70, English Only): Fundamentals of MLT & Lab Safety (10% buffered formalin, BMW plastic segregation, Westgard 1_3s rule, Abbe condenser, BSL-2 cabinets, EDTA and 3.2% citrate mechanisms, sodium fluoride enolase inhibition, Bouin’s fixative, UV 340 nm), Histopathology & Microtomy (xylene clearing, paraffin embedding at 56–58°C, alum hematoxylin mordant, H&E color kinetics, PAS stain, Perls’ Prussian blue, knife clearance angle, 95% ethanol Pap fixative, ultramicrotomy), Clinical Biochemistry (GOD-POD glucose, Jaffe’s creatinine, ALT vs AST specificity, HbA1c 8–12 week kinetics, HDL reverse cholesterol transport, uric acid gout, biuret Cu2+ reagent, bromocresol green albumin, potassium reference, CK-MB, lipase, TSH, arterial blood gas acidosis), Clinical Microbiology & AST (Gram stain reagent sequence, bacterial endospores, MacConkey agar, Ziehl-Neelsen acid-fastness, LJ egg medium, coagulase test, Kirby-Bauer disc diffusion, 10% KOH mounts, India ink Cryptococcus, HBsAg, sandwich ELISA, TCBS agar, Widal test), Parasitology (E. histolytica vs E. coli cysts, Giardia falling-leaf motility, Trichuris barrel ova, Scotch tape pinworm test, P. falciparum crescent gametocytes, thick vs thin smear, nocturnal filaria periodicity), Haematology & Blood Banking (cyanmethemoglobin principle, Neubauer 0.1 mm depth, MCV femtoliter kinetics, supravital reticulocytes, Westergren 200 mm tube, Leishman’s stain, PT/INR extrinsic pathway, iron deficiency microcytosis, reverse serum grouping, Direct Coombs test, 2–6°C PRBC storage, -18°C FFP preservation), and Urinalysis/Body Fluids (sulfosalicylic acid protein, RBC glomerulonephritis casts, CSF xanthochromia).

Non-Technical (Q71–Q100, Bilingual English & Hindi): 10 General Science + 10 Arithmetic & Reasoning + 10 General Awareness.

Written & Verified by Rohan Joshi

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