Download PDF – RRB Laboratory Assistant Grade II (MLT) CBT Mock Test 1

RRB Laboratory Assistant Grade II (MLT) CBT Mock Test 1

Q1. What is the standard concentration and volume of neutral buffered formalin used as a universal tissue fixative in histopathology?

A) 10% Neutral Buffered Formalin (4% formaldehyde)

B) 40% Pure Formaldehyde

C) 70% Ethanol

D) 5% Glutaraldehyde

Answer: (A)

10% Neutral Buffered Formalin (approx. 4% formaldehyde buffered at pH 7.2 to 7.4 with phosphates) is the universal fixative. It prevents formalin pigment (acid formaldehyde hematin) precipitation.

Q2. Under Biomedical Waste Management Rules 2016, into which color-coded container must contaminated solid plastic labware (e.g. pipette tips, plastic petri dishes, vacutainers) be discarded?

A) Yellow bag

B) Blue puncture-proof box

C) Red bag / container

D) Black general waste bin

Answer: (C)

Contaminated recyclable plastic waste such as tubing, bottles, intravenous tubes, catheters, urine bags, syringes without needles, and vacutainers is segregated into Red non-chlorinated plastic bags for autoclaving and shredding.

Q3. According to the Westgard multirule system for clinical laboratory quality control, a '1_3s' rule violation indicates:

A) Four consecutive controls exceeding 1 SD

B) Two consecutive controls exceeding 2 SD on the same side

C) A single control measurement exceeding 3 standard deviations (3 SD) from the mean (random error)

D) Ten consecutive controls falling on one side of the mean

Answer: (C)

The 1_3s rule is a rejection rule sensitive to random error where a run is rejected if a single control measurement exceeds +/- 3 standard deviations from the target mean.

Q4. Which optical part of a brightfield compound microscope concentrates and directs light onto the specimen slide?

A) Ocular eyepiece

B) Substage Condenser (Abbe condenser)

C) Objective lens

D) Iris diaphragm only

Answer: (B)

The substage condenser gathers light from the illuminator and focuses it into a bright, even cone of illumination on the specimen plane.

Q5. What is the primary function of a Class II biological safety cabinet (BSC), commonly used for aerosol-generating procedures in a BSL-2 diagnostic laboratory?

A) Sterilizing dry glassware

B) Culturing strict anaerobes only

C) Providing operator, environment, and specimen protection from moderate-risk biohazards

D) Storing volatile explosive solvents

Answer: (C)

Class II BSCs utilize laminar airflow and HEPA filtration to provide three-way protection (personnel, environment, and product) against moderate-risk pathogens (e.g. Hepatitis B, Salmonella).

Q6. Which anticoagulant acts by chelating ionized calcium (Ca2+) and is universally preferred for routine complete blood counts (CBC)?

A) Sodium Citrate (3.2%)

B) K2 / K3 EDTA (Dipotassium / Tripotassium ethylenediaminetetraacetic acid)

C) Heparin (Lithium salt)

D) Sodium Fluoride

Answer: (B)

EDTA binds calcium ions to prevent coagulation without altering erythrocyte or leucocyte morphology, making it the anticoagulant of choice for automated hematology analyzers.

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