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The DNA fragments produced by the use of restriction endonucleases can be separated by

General Science Biology Class 12th NEET UG NEET UG Preparation

Q. 3 of Quiz-1461 (Que-ID : 3396)

The DNA fragments produced by the use of restriction endonucleases can be separated by

  • polymerase chain reaction
  • gel electrophoresis
  • density gradient centrifugation
  • any of the above

Ans → (b)gel electrophoresis

Explanation → The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis

This question is taken from this post → Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET - attemp/practice


More MCQs in - Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET

Q. 1 of Quiz-1461 (Que-ID : 3394)

Biolistics (gene gun) is suitable for

  • introducing rDNA into plant cells
  • introducing rDNA into animal cells
  • disarming the pathogen vectors
  • DNA fingerprinting.

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Q. 2 of Quiz-1461 (Que-ID : 3395)

In genetic engineering experiments, restriction enzymes are used for

  • viral DNA
  • bacterial DNA
  • eukaryotic DNA
  • any type of DNA.

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Q. 3 of Quiz-1461 (Que-ID : 3396)

The DNA fragments produced by the use of restriction endonucleases can be separated by

  • polymerase chain reaction
  • gel electrophoresis
  • density gradient centrifugation
  • any of the above

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Q. 4 of Quiz-1461 (Que-ID : 3397)

Plasmids in bacterial cells are

  • extra-chromosomal DNA, which cannot replicate
  • extra-chromosomal DNA, which can . self-replicate
  • extra DNA associated with the genome
  • extra DNA, associated with the genome, but cannot replicate.

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Q. 5 of Quiz-1461 (Que-ID : 3398)

The DNA polymerase enzyme used in PCR is obtained from

  • Thermus aquaticus
  • Escherichia coli
  • Agrobacterium tumefaciens
  • Salmonella typhimurium

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Q. 6 of Quiz-1461 (Que-ID : 3399)

While isolating DNA from bacteria, which of the following enzymes is not used?

  • Lysozyme
  • Ribonuclease
  • Deoxyribonuclease
  • Protease

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Q. 7 of Quiz-1461 (Que-ID : 3400)

Significance of ‘heat shock’ method in bacterial transformation is to facilitate

  • binding of DNA to the cell wall
  • uptake of DNA through membrane transport proteins
  • uptake of DNA through transient pores in the bacterial cell wall
  • expression of antibiotic resistance gene

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Q. 8 of Quiz-1461 (Que-ID : 3401)

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

  • Denaturation of template DNA
  • Annealing of primers to template DNA
  • Extension of primer end on the template DNA
  • All of the above

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Q. 9 of Quiz-1461 (Que-ID : 3402)

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

  • DNA can be seen in visible light
  • DNA can be seen without staining in visible light
  • Ethidium bromide stained DNA can be seen in visible light
  • Ethidium bromide stained DNA can be seen under exposure to UV light

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Q. 10 of Quiz-1461 (Que-ID : 3403)

Restriction’ in Restriction enzyme refers to

  • cleaving ofphosphodiester bond in DNA by the enzyme
  • cutting of DNA at specific position only
  • prevention of the multiplication of bacteriophage in bacteria
  • all of the above

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Q. 11 of Quiz-1461 (Que-ID : 3404)

The role of DNA ligase in the construction of a recombinant DNA molecule is

  • formation of phosphodiester bond between two DNA fragments
  • formation of hydrogen bonds between sticky ends of DNA fragments
  • ligation of all purine and pyrimidine bases
  • none of the above

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Q. 12 of Quiz-1461 (Que-ID : 3405)

In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be

  • both transformants and non-transformants cannot survive
  • both transformants and non-transformants can survive.
  • transformants only and not the non-transformants can survive
  • transformants cannot survive, but non-transformants can not.

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Q. 13 of Quiz-1461 (Que-ID : 3406)

DNA element with ability to change its position is called

  • Cistron
  • Transposon
  • Intron
  • Ricon

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Q. 14 of Quiz-1461 (Que-ID : 3407)

Viral genome incroporatcd into host DNA is called:

  • Prophase
  • Protophage
  • Bacteriophage
  • None of these

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Q. 15 of Quiz-1461 (Que-ID : 3408)

Triticale, first man made cereal crop, has been obtained by crossing wheat with:

  • Rye
  • Pearl millet
  • Sugarcane
  • Barley

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Q. 16 of Quiz-1461 (Que-ID : 3409)

Golden rice is a promising transgenic crop. When released for cultivation, it will help in :

  • High lysin content
  • Pest resistance
  • High protien content
  • High vitamin A content

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Q. 17 of Quiz-1461 (Que-ID : 3410)

Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from

  • Plasmids
  • AH prokaryotic cells
  • Bacteriophages
  • Bacterial cells

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Q. 18 of Quiz-1461 (Que-ID : 3411)

Restriction endonuclease cuts

  • One stand of DNA at specific site
  • Both strands of DNA
  • Both strands of DNA at any site
  • Single strand of RNA

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Q. 19 of Quiz-1461 (Que-ID : 3412)

Plasmid is a

  • Fungus
  • Plasmid
  • Part of Plasma membrane
  • Extra chromosomal DNA in bacterial cell

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Q. 20 of Quiz-1461 (Que-ID : 3413)

Two microbes found tb be very useful in Genetic Engineering are

  • Excherichia coli and Agrobacterium tumefaciens
  • Vibrio cholarae and a tailed bacteriophage
  • Diplococcus sp. and Pseudomonas sp.
  • Crown gall bacterium and Caenorhabdities elegans

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Q. 21 of Quiz-1461 (Que-ID : 3414)

DNA obtained for DNA finger printing:

  • W.B.C
  • Hair root cells
  • Body fluids
  • None of these

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Q. 22 of Quiz-1461 (Que-ID : 3415)

Who discovered Restricted endonucleas enzymes?

  • Smith and Nathane
  • Baiger
  • Waxman
  • Fleming

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Q. 23 of Quiz-1461 (Que-ID : 3416)

Two microbes found to be very useful in genetic, engineering are

  • Escherichiya Coli and Agrobacterium tumefaciens
  • Virbio chloerae and tailed bacteriophage
  • Diplococcus sp and pseudomonas sp
  • None of these

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Q. 24 of Quiz-1461 (Que-ID : 3417)

Transgenic plants are develop by

  • Introducing of foreign fenes
  • Clone and genetically modified genes
  • Genetic engineering
  • Purified genes

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Q. 25 of Quiz-1461 (Que-ID : 3418)

Transgenic plants are develop by

  • Introducing of foreign fenes
  • Clone and genetically modified genes
  • Genetic engineering
  • Purified genes

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Q. 5 of Quiz- (Que-ID : 2673)

What is the percent of formed elements in blood

  • 55
  • 45
  • 35
  • 95

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Q. 2 of Quiz- (Que-ID : 2895)

After ovulation Graafian follicle regreses into

  • corpus atresia
  • corpus callosum
  • corpus luteum
  • corpus albicans

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Q. 2 of Quiz- (Que-ID : 2945)

The first genetic material could be

  • protein
  • cabohydrates
  • DNA
  • RNA

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Q. 24 of Quiz- (Que-ID : 3342)

Which of the following is affected by the infection of Wuchereria bancrofti ?

  • Lymphatic vessels
  • Respiratory system
  • Nervous system
  • Blood circulation

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Q. 16 of Quiz- (Que-ID : 3359)

Multiple ovulation embryo transfer technology is related to

  • transfer of super embryo
  • transfer of super eggs
  • super ovulation and embryo transfer
  • both (a) and (b)

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Q. 5 of Quiz- (Que-ID : 3248)

Choose the correct pair.

  • Hippocampus – 3-chambered heart
  • Rana – 2-chambered heart
  • Crocodilus – 4-chambered heart
  • Pavo – 3-chambered heart

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Q. 25 of Quiz- (Que-ID : 2743)

What is not part of sexual cycle of fungi

  • Plasmogamy
  • Mitosis
  • Karyogamy
  • Meiosis

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Q. 20 of Quiz- (Que-ID : 2989)

ANF can cause

  • Vasoconstriction
  • Vasodilation
  • Both a and b
  • None of the above

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Q. 13 of Quiz- (Que-ID : 3531)

Minimata disease is caused by

  • ByChromium
  • By Cadmium
  • By Methyl mercury
  • By Radioactive elements

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Q. 9 of Quiz- (Que-ID : 3227)

Thors and spines are

  • homologous organs
  • analogous organs
  • thron is homologous while spine is analogous
  • spine is homologous while is analogous

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Q. 8 of Quiz- (Que-ID : 3526)

Fluoride pollution mainly affect

  • Teeth
  • Kidney
  • Brain
  • Heart

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Q. 25 of Quiz- (Que-ID : 2818)

Which is not part of brain stem

  • Pons
  • Medulla
  • Mid brain
  • Cerebellum

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Q. 22 of Quiz- (Que-ID : 2635)

Enamel is originated from which cell

  • Amiloblast cell
  • Odontioblast cell
  • Chief cell
  • Peptic cell

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Q. 17 of Quiz- (Que-ID : 3085)

Which of the following is a 4-carbon compound?

  • Oxaloacetic acid
  • Phosphoglyceric acid
  • Ribulose biphosphate
  • Phosphoenolpyruvate

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Q. 13 of Quiz- (Que-ID : 3156)

NADP contains vitamin ______

  • B1
  • B2
  • B3
  • B12

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Q. 16 of Quiz- (Que-ID : 3084)

Kranz anatomy is a feature of

  • C3 plants
  • CAM plants
  • C4 plants
  • All of these

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Directions : (Q.1 - Q.15 Physics part)

Q. 1 of Quiz- (Que-ID : 558)

Galvanized iron is coated with a thin coating of-

  • copper
  • zinc
  • aluminium
  • white lead

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Q. 4 of Quiz- (Que-ID : 3522)

Acid Rain comes under which type of pollution?

  • Water pollution
  • Air pollution
  • Soil pollution
  • Sound Pollution

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Q. 14 of Quiz- (Que-ID : 3257)

Male mosquitoes usually feed on

  • Garbage
  • Human blood
  • Flower sap
  • All of the above

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Q. 3 of Quiz- (Que-ID : 3071)

The primary carbon dioxide acceptor in C4 cycle is

  • malic acid
  • phosphoenol pyruvate
  • rubisco
  • aspartic acid

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Q. 2 of Quiz- (Que-ID : 2945)

The first genetic material could be

  • protein
  • cabohydrates
  • DNA
  • RNA

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Q. 18 of Quiz- (Que-ID : 3061)

Respiratory enzymes are located in

  • mitochondrial matrix
  • Cristae
  • perimitochondrial space
  • outer membrane

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Q. 25 of Quiz- (Que-ID : 2693)

What condition occurs when not enough oxygen reaches heart muscle

  • High bp
  • Low bp
  • Angina
  • Heart failure

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Q. 8 of Quiz- (Que-ID : 3051)

Herkogamy is a contrivance for

  • allogamy
  • autogamy
  • chasmogamy
  • cleistogamy

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Q. 10 of Quiz- (Que-ID : 2653)

What is the inspiratory reserve volume

  • 2500-3000 ml
  • 500 ml
  • 1000-1100 ml
  • None of the above

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Q. 17 of Quiz- (Que-ID : 2935)

Phenotypic and genotypic ratio is similar in case of

  • complete dominance
  • incomplete dominance
  • over dominance
  • epistasis

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Q. 25 of Quiz- (Que-ID : 3368)

Black rust of wheat is caused by

  • Puccinia
  • Albugo
  • Ustilago
  • Cystopus

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Q. 3 of Quiz- (Que-ID : 3146)

Insulin is made up of _______ and _______.

  • glucose and fructose
  • glucose and fructose
  • fructose and mannose
  • mannose and glucose

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Q. 21 of Quiz- (Que-ID : 3114)

The nitrogen exists as two nitrogen atoms joined by

  • Di covalent bonds
  • tetra covalent bonds
  • triple covalent bonds
  • monocovalent bond

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Q. 11 of Quiz- (Que-ID : 3179)

The ciliated epithelium in our body may be found in

  • trachea
  • ureter
  • bile duct
  • intestines

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Q. 13 of Quiz- (Que-ID : 584)

In a freezer ice cube will be formed more quickly in trays made of

  • rubber
  • plastic
  • aluminium
  • wood

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Q. 10 of Quiz- (Que-ID : 3003)

what type of plant is formed when colchicine is used in the process of development of Raphanobrassica?

  • Autotetraploid
  • Haploid
  • Triploid
  • Allotetraploid

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Q. 20 of Quiz- (Que-ID : 3238)

The embryo in sunflower has

  • one cotyledon
  • two cotyledons
  • many cotyledons
  • no cotyledon

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Q. 20 of Quiz- (Que-ID : 3363)

Which of the following plays a role in indigenous system of medicine ?

  • Plant breeding
  • Fisheries
  • Apiculture
  • MOET

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Q. 25 of Quiz- (Que-ID : 3268)

Which one of the following groups of animals is bilaterally symmetrical and triploblastic

  • Aschelminthes (round worms)
  • Ctenophores
  • Sponges
  • Coelenterates (Cnidarians)

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Q. 20 of Quiz- (Que-ID : 3538)

Permissible noise levels in a residential area at night time is

  • 35 dB
  • 40 dB
  • 45 dB
  • 50 dB

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Q. 1 of Quiz- (Que-ID : 3219)

In tamarind, the pinnate leaf is

  • bipinnate
  • tripinnate
  • paripinnate
  • imparipinnate

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Q. 9 of Quiz- (Que-ID : 3202)

Stem grows in girth due to

  • outer cortical
  • epidermis
  • vascular cambium
  • phellogen

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Q. 25 of Quiz- (Que-ID : 2968)

Which out of the following statements is incorrect ?

  • Genetic code is ambiguous.
  • Genetic code is deqenerate
  • Genetic code is universal
  • Genetic code is non-overlanning.

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Q. 19 of Quiz- (Que-ID : 3362)

The term ‘apiculture’ refers to

  • tissue culture
  • pisciculture
  • bee-keeping
  • animal-keeping

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Q. 19 of Quiz- (Que-ID : 3362)

The term ‘apiculture’ refers to

  • tissue culture
  • pisciculture
  • bee-keeping
  • animal-keeping

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Q. 13 of Quiz- (Que-ID : 2706)

Which among the following are involuntary muscles

  • Cardiac
  • Skeletal
  • Smooth
  • Both a and c

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Q. 4 of Quiz- (Que-ID : 3372)

Organic farming includes

  • use of fertilisers and pesticides of biological origin
  • IPM (Integrated Pest Management)
  • locally developed pest resistant varieties
  • all of these

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