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The DNA fragments produced by the use of restriction endonucleases can be separated by
General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 3 of Quiz-1461 (Que-ID : 3396)
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Ans → (b)gel electrophoresis
Explanation → The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
This question is taken from this post → Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET - attemp/practice
More MCQs in - Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET
Q. 1 of Quiz-1461 (Que-ID : 3394)
Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
Q. 2 of Quiz-1461 (Que-ID : 3395)
In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
Q. 3 of Quiz-1461 (Que-ID : 3396)
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Q. 4 of Quiz-1461 (Que-ID : 3397)
Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
Q. 5 of Quiz-1461 (Que-ID : 3398)
The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
Q. 6 of Quiz-1461 (Que-ID : 3399)
While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
Q. 7 of Quiz-1461 (Que-ID : 3400)
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Q. 8 of Quiz-1461 (Que-ID : 3401)
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
Q. 9 of Quiz-1461 (Que-ID : 3402)
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
Q. 10 of Quiz-1461 (Que-ID : 3403)
Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
Q. 11 of Quiz-1461 (Que-ID : 3404)
The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
Q. 12 of Quiz-1461 (Que-ID : 3405)
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
Q. 13 of Quiz-1461 (Que-ID : 3406)
DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
Q. 14 of Quiz-1461 (Que-ID : 3407)
Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
Q. 15 of Quiz-1461 (Que-ID : 3408)
Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
Q. 16 of Quiz-1461 (Que-ID : 3409)
Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
Q. 17 of Quiz-1461 (Que-ID : 3410)
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
Q. 18 of Quiz-1461 (Que-ID : 3411)
Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
Q. 19 of Quiz-1461 (Que-ID : 3412)
Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
Q. 20 of Quiz-1461 (Que-ID : 3413)
Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
Q. 21 of Quiz-1461 (Que-ID : 3414)
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Q. 22 of Quiz-1461 (Que-ID : 3415)
Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
Q. 23 of Quiz-1461 (Que-ID : 3416)
Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
Q. 24 of Quiz-1461 (Que-ID : 3417)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Q. 25 of Quiz-1461 (Que-ID : 3418)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
More MCQs In - General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 2 of Quiz- (Que-ID : 2820)
Which of the following options shows bisexual animals only?
- Amoeba, sponge, leech
- Sponge, cockroach, Amoeba
- Earthworm, sponge, leech
- Tapeworm, earthworm, honeybee
Q. 10 of Quiz- (Que-ID : 3428)
Somatic hybridization can be done by:
- By Protoplast fusion
- By Haploid anther
- By cell culture
- By Pollen culture
Q. 24 of Quiz- (Que-ID : 3167)
The introduction of t-DNA into plants involves
- Altering the pH of the soil, then heat shocking the plants
- Exposing the plants to cold for a brief period
- Allowing the plant roots to stand in water
- Infection of the plant by Agrobacterium tumefaciens
Q. 20 of Quiz- (Que-ID : 3538)
Permissible noise levels in a residential area at night time is
- 35 dB
- 40 dB
- 45 dB
- 50 dB
Q. 18 of Quiz- (Que-ID : 3236)
Blue dye is obtained from the leaves of
- Indigofera tinctoria
- Opium
- Aloe
- Delbergia sissoo
Q. 8 of Quiz- (Que-ID : 3101)
Choose the correct function of magnesium
- It is a constituent of several coenzyme
- It activates the enzymes of respiration and photosynthesis.
- It activates enzyme catalase
- It helps in maintaining anion-cation balance
Q. 1 of Quiz- (Que-ID : 2644)
Which among the following does not exchange o2 with CO2 through simple diffusion
- Earthworms
- Earthworms
- Sponges
- Flatworms
Q. 12 of Quiz- (Que-ID : 3255)
Which kind of symmetry occurs in sea anemone?
- Bilateral
- Radial
- Asymmetry
- None of these
Q. 25 of Quiz- (Que-ID : 2668)
which among the following is responsible to shift o2 dissociation curve right
- High Pco2
- Low pco2
- Low H+
- None of the above
Q. 1 of Quiz- (Que-ID : 2769)
Wheat belongs to division
- angiospermae
- gymnospermae
- Poach
- None of the above
Q. 2 of Quiz- (Que-ID : 3295)
The phenomenon ‘ontogeny repeats phylogeny’ is explained by
- recapitualtion theory
- Inheritcance theory
- mutation theory
- natural selection theory.
Q. 20 of Quiz- (Que-ID : 3538)
Permissible noise levels in a residential area at night time is
- 35 dB
- 40 dB
- 45 dB
- 50 dB
Q. 11 of Quiz- (Que-ID : 3004)
Meiosis occurs for the human female in ______.
- Pancreas
- Liver
- Ovary
- Kidney
Q. 8 of Quiz- (Que-ID : 2901)
Which part of the sperm plays an important role in penetrating the egg membrane ?
- Allosome
- Tail
- Autosome
- Acrosome
Q. 4 of Quiz- (Que-ID : 2722)
Name the classification based on genetic and evolutionary relationship
- Phonetics
- Biosynthesis
- Numerical taxonomy
- Cladistics
Q. 20 of Quiz- (Que-ID : 3538)
Permissible noise levels in a residential area at night time is
- 35 dB
- 40 dB
- 45 dB
- 50 dB
Q. 2 of Quiz- (Que-ID : 3095)
Function of zinc is
- Closing of stomata
- Biosynthesis of 3-IAA
- Synthesis of chlorophyll
- Oxidation of carbohydrate
Q. 2 of Quiz- (Que-ID : 3470)
Soil particle determine the character of
- Organisation
- Biomass
- Area capacity
- Soil plants
Q. 12 of Quiz- (Que-ID : 3080)
Translocation of sugar in flowering plants occurs in the form of
- glucose
- sucrose
- starch
- maltose
Q. 7 of Quiz- (Que-ID : 2725)
In which form fungi store food material
- Lipid
- Starch
- Oil and glycogen
- Protein
Q. 24 of Quiz- (Que-ID : 3217)
Which of the following provide maximum mechanical strength to a tree trunk.
- Heart wood
- Sap wood
- Cork
- Late wood
Q. 9 of Quiz- (Que-ID : 2877)
Condoms are one of the most popular contraceptives because of the following reasons.
- These are effective barriers for insemination
- They do not interfere with coital act
- These help in reducing the risk of STDs
- All of the above
Q. 7 of Quiz- (Que-ID : 3225)
In Lathyrus, the leaves are modified into
- thorns
- cladodes
- tendrils
- spines
Q. 10 of Quiz- (Que-ID : 1587)
The process of breakdown of glycogen to blood glucose is … . .
- Gluconeogenesis
- Glycogenesis
- Thermogenesis
- Glycogenolysis
Q. 19 of Quiz- (Que-ID : 3537)
Act formulated in 1996 was
- Insecticide Act
- Air (Prevention and Control of Pollution) Act
- Water (Prevention and control of Pollution) Act
- Environment protection Act
Q. 3 of Quiz- (Que-ID : 2971)
Which among the following is not uricootelic
- Mammal
- Reptiles
- Birds
- Snails
Q. 5 of Quiz- (Que-ID : 2998)
Karyokinesis is the term used for
- division of cytoplasm
- division of nucleoplasm
- division of nucleus
- separation of daughter chromosomes
Q. 15 of Quiz- (Que-ID : 3308)
Genetic drift operates only in
- larger populations
- Mendelian populations
- island populations
- smaller populations.
Q. 14 of Quiz- (Que-ID : 2627)
Which is not present in bile juice
- Bile pigments
- Enzymes
- Bile salts
- Cholesterol
Q. 4 of Quiz- (Que-ID : 2947)
Who amongst the following scientist had no contribution in the development of the double helix model for the structure of DN A ?
- Rosalind Franklin
- Maurice Wilkins
- Erwin Chargaff
- Meselson and Stahl
Q. 9 of Quiz- (Que-ID : 3252)
Which one of the following groups of animals is correctly matched with its one characteristic feature without even a single exception?
- Mammalia : give birth to young ones
- Reptilia : possess 3-chambered heart with one incompletely divided ventricle
- Chordata : possess a mouth provided with an upper and a lower jaw
- Chondrichthyes : possess cartilaginous endoskeleton
Q. 14 of Quiz- (Que-ID : 2807)
Which of the following is not related to the autonomic nervous system?
- Peristalsis
- Digestion
- Excretion
- Memory and learning
Q. 19 of Quiz- (Que-ID : 3537)
Act formulated in 1996 was
- Insecticide Act
- Air (Prevention and Control of Pollution) Act
- Water (Prevention and control of Pollution) Act
- Environment protection Act
Q. 8 of Quiz- (Que-ID : 3251)
Osteichthyes belongs to
- class amphibia
- super class pisces
- super class tetrapoda
- division agnatha
Q. 18 of Quiz- (Que-ID : 3461)
In which one of the following habitats, does the diurnal temperature of soil surface view most?
- Shrub land
- Forest
- Desert
- Grassland
Q. 9 of Quiz- (Que-ID : 2677)
Where does erythropoiesis in adult takes place
- Bone marrow
- Spleen
- Thymus
- None of the above
Q. 4 of Quiz- (Que-ID : 3122)
Two cells A and B are contiguous. Cell A has osmotic pressure 10 atm, turgor pressure 7 atm and diffusion pressure deficit 3 atm. Cell B has osmotic pressure 8 atm, turgor pressure 3 atm and diffusion pressure deficit 5 atm. The result will be
- No movement of water
- Movement of water from cell B to A
- Movement of water from cell A to B
- Equilibrium between the two
Q. 19 of Quiz- (Que-ID : 3362)
The term ‘apiculture’ refers to
- tissue culture
- pisciculture
- bee-keeping
- animal-keeping
Q. 8 of Quiz- (Que-ID : 3376)
Which one of the following can be used as biofertiliser in cotton field ?
- Azolla-Anabaena
- Streptococcus
- Azospirillum
- Azotobacter chroococcum
Q. 16 of Quiz- (Que-ID : 2884)
Which of the following pairs contributes to an increase in population ?
- Natality and immigration
- Mortality and emigration
- Natality and emigration
- Mortality and immigration
Q. 25 of Quiz- (Que-ID : 3143)
ψp denotes to
- Water potential
- Negative potential
- Pressure potential
- Solute potential
Q. 7 of Quiz- (Que-ID : 3425)
Bt Toxin is :
- Intracellular lipid
- Intracellular crystal protein
- Extracellular Crystal protein
- Lipid
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