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Significance of ‘heat shock’ method in bacterial transformation is to facilitate
General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 7 of Quiz-1461 (Que-ID : 3400)
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Ans → (c)uptake of DNA through transient pores in the bacterial cell wall
Explanation → uptake of DNA through transient pores in the bacterial cell wall
This question is taken from this post → Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET - attemp/practice
More MCQs in - Biotechnology: Principles and Processes - Biology (Class 12th) Revision Test | MCQs for NEET
Q. 1 of Quiz-1461 (Que-ID : 3394)
Biolistics (gene gun) is suitable for
- introducing rDNA into plant cells
- introducing rDNA into animal cells
- disarming the pathogen vectors
- DNA fingerprinting.
Q. 2 of Quiz-1461 (Que-ID : 3395)
In genetic engineering experiments, restriction enzymes are used for
- viral DNA
- bacterial DNA
- eukaryotic DNA
- any type of DNA.
Q. 3 of Quiz-1461 (Que-ID : 3396)
The DNA fragments produced by the use of restriction endonucleases can be separated by
- polymerase chain reaction
- gel electrophoresis
- density gradient centrifugation
- any of the above
Q. 4 of Quiz-1461 (Que-ID : 3397)
Plasmids in bacterial cells are
- extra-chromosomal DNA, which cannot replicate
- extra-chromosomal DNA, which can . self-replicate
- extra DNA associated with the genome
- extra DNA, associated with the genome, but cannot replicate.
Q. 5 of Quiz-1461 (Que-ID : 3398)
The DNA polymerase enzyme used in PCR is obtained from
- Thermus aquaticus
- Escherichia coli
- Agrobacterium tumefaciens
- Salmonella typhimurium
Q. 6 of Quiz-1461 (Que-ID : 3399)
While isolating DNA from bacteria, which of the following enzymes is not used?
- Lysozyme
- Ribonuclease
- Deoxyribonuclease
- Protease
Q. 7 of Quiz-1461 (Que-ID : 3400)
Significance of ‘heat shock’ method in bacterial transformation is to facilitate
- binding of DNA to the cell wall
- uptake of DNA through membrane transport proteins
- uptake of DNA through transient pores in the bacterial cell wall
- expression of antibiotic resistance gene
Q. 8 of Quiz-1461 (Que-ID : 3401)
Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
- Denaturation of template DNA
- Annealing of primers to template DNA
- Extension of primer end on the template DNA
- All of the above
Q. 9 of Quiz-1461 (Que-ID : 3402)
Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
- DNA can be seen in visible light
- DNA can be seen without staining in visible light
- Ethidium bromide stained DNA can be seen in visible light
- Ethidium bromide stained DNA can be seen under exposure to UV light
Q. 10 of Quiz-1461 (Que-ID : 3403)
Restriction’ in Restriction enzyme refers to
- cleaving ofphosphodiester bond in DNA by the enzyme
- cutting of DNA at specific position only
- prevention of the multiplication of bacteriophage in bacteria
- all of the above
Q. 11 of Quiz-1461 (Que-ID : 3404)
The role of DNA ligase in the construction of a recombinant DNA molecule is
- formation of phosphodiester bond between two DNA fragments
- formation of hydrogen bonds between sticky ends of DNA fragments
- ligation of all purine and pyrimidine bases
- none of the above
Q. 12 of Quiz-1461 (Que-ID : 3405)
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
- both transformants and non-transformants cannot survive
- both transformants and non-transformants can survive.
- transformants only and not the non-transformants can survive
- transformants cannot survive, but non-transformants can not.
Q. 13 of Quiz-1461 (Que-ID : 3406)
DNA element with ability to change its position is called
- Cistron
- Transposon
- Intron
- Ricon
Q. 14 of Quiz-1461 (Que-ID : 3407)
Viral genome incroporatcd into host DNA is called:
- Prophase
- Protophage
- Bacteriophage
- None of these
Q. 15 of Quiz-1461 (Que-ID : 3408)
Triticale, first man made cereal crop, has been obtained by crossing wheat with:
- Rye
- Pearl millet
- Sugarcane
- Barley
Q. 16 of Quiz-1461 (Que-ID : 3409)
Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
- High lysin content
- Pest resistance
- High protien content
- High vitamin A content
Q. 17 of Quiz-1461 (Que-ID : 3410)
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
- Plasmids
- AH prokaryotic cells
- Bacteriophages
- Bacterial cells
Q. 18 of Quiz-1461 (Que-ID : 3411)
Restriction endonuclease cuts
- One stand of DNA at specific site
- Both strands of DNA
- Both strands of DNA at any site
- Single strand of RNA
Q. 19 of Quiz-1461 (Que-ID : 3412)
Plasmid is a
- Fungus
- Plasmid
- Part of Plasma membrane
- Extra chromosomal DNA in bacterial cell
Q. 20 of Quiz-1461 (Que-ID : 3413)
Two microbes found tb be very useful in Genetic Engineering are
- Excherichia coli and Agrobacterium tumefaciens
- Vibrio cholarae and a tailed bacteriophage
- Diplococcus sp. and Pseudomonas sp.
- Crown gall bacterium and Caenorhabdities elegans
Q. 21 of Quiz-1461 (Que-ID : 3414)
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Q. 22 of Quiz-1461 (Que-ID : 3415)
Who discovered Restricted endonucleas enzymes?
- Smith and Nathane
- Baiger
- Waxman
- Fleming
Q. 23 of Quiz-1461 (Que-ID : 3416)
Two microbes found to be very useful in genetic, engineering are
- Escherichiya Coli and Agrobacterium tumefaciens
- Virbio chloerae and tailed bacteriophage
- Diplococcus sp and pseudomonas sp
- None of these
Q. 24 of Quiz-1461 (Que-ID : 3417)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
Q. 25 of Quiz-1461 (Que-ID : 3418)
Transgenic plants are develop by
- Introducing of foreign fenes
- Clone and genetically modified genes
- Genetic engineering
- Purified genes
More MCQs In - General Science Biology Class 12th NEET UG NEET UG Preparation
Q. 21 of Quiz- (Que-ID : 3414)
DNA obtained for DNA finger printing:
- W.B.C
- Hair root cells
- Body fluids
- None of these
Q. 18 of Quiz- (Que-ID : 1980)
RBCs are generally destroyed in the liver, while WBCs are destroyed in-
- Plasma
- Lymph
- Inside various cells of body
- Out side the blood stream
Q. 18 of Quiz- (Que-ID : 2961)
How far is each base pair from the next one in DNA double helix model ?
- 2 nm
- 3.4 nm
- 34 nm
- 0.34 nm
Q. 24 of Quiz- (Que-ID : 3492)
Best type of pyramid which is always true is:
- Pyramid or energy
- Pyramid of Biomass
- Pyramid of number
- Both (a) and (c)
Q. 9 of Quiz- (Que-ID : 2827)
In flowering plants, both male and female gametes are non-motile. The method to bring them together for fertilisation is
- water
- air
- pollination
- apomixis
Q. 1 of Quiz- (Que-ID : 3444)
For human population, the age pyramids generally show age distribution of
- Only males
- Only females
- Of males and females
- None of the above
Q. 11 of Quiz- (Que-ID : 3079)
Discovery of Emerson effect has already shown the
- two distinct photochemical reactions
- light and dark reactions of photosynthesis
- photophosphorylation
- photorespiration
Q. 11 of Quiz- (Que-ID : 3304)
Which one of the following scientist’s name is correctly matched with the theory put forth by him ?
- de Vries – Theory of natural selection
- Darwin – Theory of pangenesis
- Weismann – Theory of continuity of germplasm
- Pasteur – Theory of inheritance of acquired charcters
Q. 7 of Quiz- (Que-ID : 78)
White phosphorous is generally kept under-
- Alcohol
- Water
- glycerine
- ether
Q. 15 of Quiz- (Que-ID : 2708)
Which joint is present between humerus and pectoral girdle
- Hinge
- Ball and socket
- Pivot joint
- Condyloid
Q. 18 of Quiz- (Que-ID : 3286)
Bryophytes are called amphibians of plant kingdom because
- These plants live in soil and depend on marine organisms for asexual reproduction
- These plants live in soil and depend on water for sexual reproduction.
- These plants live in water and depend on land animals for sexual reproduction
- These plants live near water bodies.
Q. 23 of Quiz- (Que-ID : 2966)
They year 2003 was celebrated as the 50th anniversary of discovery of
- transposons by Barbare Me Clintock
- structure of DNA by Watson and Crick
- Mendel’s laws of inheritance
- biotechnology by Kary Muliis.
Q. 18 of Quiz- (Que-ID : 2736)
Which of the feature mycoplasma does not have
- Pleomorphic
- Lack cell wall
- All the above
- Can?t live w/o O2
Q. 23 of Quiz- (Que-ID : 3016)
A cell plate is laid down during
- Cytokinesis
- Interphase
- Karyokinesis
- None of these
Q. 11 of Quiz- (Que-ID : 3004)
Meiosis occurs for the human female in ______.
- Pancreas
- Liver
- Ovary
- Kidney
Q. 6 of Quiz- (Que-ID : 2849)
From among the sets of terms given below, identify those that are associated with the gynoecium
- Stigma, ovule, embryo sac, placenta
- Thalamus, pistil, style, ovule
- Ovule, ovary, embryo sac, tapetum
- Ovule, stamen, ovary, embryo sac
Q. 16 of Quiz- (Que-ID : 3184)
Tendons connect the following
- bone to bone
- muscle to muscle
- cartilage to muscle
- bone to muscle
Q. 10 of Quiz- (Que-ID : 2953)
The RNA polymerase holocnzyme transcribes
- the promoter, structural gene and the terminator region
- the promoter and the terminator region
- the structural gene and the terminator region
- the structural gene only.
Q. 4 of Quiz- (Que-ID : 3072)
The law of limiting factor was given by
- Calvin
- Blackman
- Priestley
- None of these
Q. 17 of Quiz- (Que-ID : 2910)
spot the odd one out from the following structures with reference to the male reproductive system
- Rate testis
- Epididymis
- Vasa efferentia
- Isthmus
Q. 25 of Quiz- (Que-ID : 2918)
At what stage of life is oogenesis initiated in a human female ?
- At puberty
- During menarch
- During menopause
- During embryonic development
Q. 1 of Quiz- (Que-ID : 2994)
Golgi complex and endoplasmic reticulum cannot be seen during
- late telophase
- late prophase
- early anaphase
- late metaphase
Q. 1 of Quiz- (Que-ID : 3519)
Which chemical substance affects the Ozone Layer?
- Chlorofluorocarbon
- Chlorine
- Hexafluorocarbon
- Molecular Carbon
Q. 1 of Quiz- (Que-ID : 3069)
The rate of photosynthesis is controlled by
- the rate of light reaction
- the rate of dark reaction
- the rates of both light and dark reactions
- none of the above
Q. 15 of Quiz- (Que-ID : 3233)
The axillary buds arise
- endogenously from the pericycle
- exogenously from the tissues of the shoot apex
- endogenously from the cambium tissues
- exogenously from the innermost layers of cortex
Q. 2 of Quiz- (Que-ID : 3320)
Which one of the following disease is non – communicable ?
- Diphtheria
- Flu
- Cancer
- Malaria
Q. 12 of Quiz- (Que-ID : 3430)
Which one of the following is a step of PCR?
- Denaturation
- Annealing
- Extension
- All of these
Q. 6 of Quiz- (Que-ID : 2849)
From among the sets of terms given below, identify those that are associated with the gynoecium
- Stigma, ovule, embryo sac, placenta
- Thalamus, pistil, style, ovule
- Ovule, ovary, embryo sac, tapetum
- Ovule, stamen, ovary, embryo sac
Q. 5 of Quiz- (Que-ID : 2973)
Which requires min water for removal from body
- Ammonia
- Urea
- Uric acid
- None of the above
Q. 5 of Quiz- (Que-ID : 3498)
Which type of living organisms given below are listed in Red Data Book”?
- Rare species
- Rare and endangered species
- Endangered species
- None of these
Q. 13 of Quiz- (Que-ID : 2781)
The study of fish is called
- Ophiology
- Herpetology
- ichthyology
- pisiculture
Q. 10 of Quiz- (Que-ID : 1719)
The organism that lives at the cost of another living organism is called-
- Aphrodite
- saprophyte
- epiphyte
- parasite
Q. 4 of Quiz- (Que-ID : 3047)
Which enzyme catalyses the reaction below? Pyruvic acid → Carbon dioxide + Ethanol
- Pyruvic acid decarboxylase
- Alcohol dehydrogenase
- None of these
- Both of these
Q. 23 of Quiz- (Que-ID : 3166)
Anti-parallel strands of a DNA molecule mean that
- One strand turns anticlockwise
- Phosphate groups at the start of two DNA strands (poles) are in opposite position
- Phosphate groups of two DNA strands at their ends share the same position
- One strand turns clockwise
Q. 5 of Quiz- (Que-ID : 2923)
What is the probability of production of dwarf offsprings in a cross betweeen two heterozygous tall pea plants ?
- Zero
- 50%
- 25%
- 100%
Q. 6 of Quiz- (Que-ID : 2949)
Control of gene expression takes place at the level of
- DNA-replication
- transcription
- translation
- none of the above
Q. 16 of Quiz- (Que-ID : 2834)
There are various types of reproduction. The type of reproduction adopted by an organism depends on
- the habitat and morphology of the organism
- morphology of the organism
- morphology and physiology of the organism
- the organisms habitat, physiology and genetic makeup.
Q. 8 of Quiz- (Que-ID : 2701)
Which among the following is auto immune disorder
- Tetany
- Arthritis
- Myasthenia gravis
- Gout
Q. 12 of Quiz- (Que-ID : 2905)
The function of the secretion of prostate gland is to
- inhibit sperm activity
- attract sperms
- stimulate sperm activity
- none of these.
Q. 23 of Quiz- (Que-ID : 2966)
They year 2003 was celebrated as the 50th anniversary of discovery of
- transposons by Barbare Me Clintock
- structure of DNA by Watson and Crick
- Mendel’s laws of inheritance
- biotechnology by Kary Muliis.
Q. 22 of Quiz- (Que-ID : 3065)
Most of the energy of the carbohydrates is released by oxidation when
- Pyruvic acid is converted into CO2 and H2O
- Pyruvic acid is converted into acetyl Co-A
- Sugar is converted into pyruvic acid
- Glucose is converted into alcohol and CO2
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