A) เคเคพเคฐเฅเคฌเคจ เคเฅเคเฅเคฐเคพเคเฅเคฒเฅเคฐเคพเคเคก
B) เคเคพเคเคฒเฅเคจ / เคกเคพเคเคฎเคฟเคฅเคพเคเคฒเคฌเฅเคเคเฅเคจ (Xylene)
C) เคคเคฐเคฒ เคชเฅเคฐเคพเคซเคฟเคจ เคคเฅเคฒ
D) เคเคฅเคฟเคฒเฅเคจ เคเฅเคฒเคพเคเคเฅเคฒ
Answer: (B)
Xylene is widely used because it is miscible with the dehydrating alcohol and paraffin wax. It is not the only clearing agent; suitability depends on the tissue and laboratory protocol.
Prolonged immersion in xylene hardens tissues severely, rendering fibrous and muscular specimens brittle, which leads to shattering and chattering during section cutting.
Routine paraffin wax used in diagnostic histopathology has an optimal melting point of 56ยฐC to 58ยฐC (or up to 60ยฐC in tropical climates), balancing block stability with tissue softness.
Wax baths are strictly regulated at 2ยฐCโ3ยฐC above the wax melting point (typically 58ยฐCโ60ยฐC). Excess heat hardens and distorts tissues, while lower temperatures cause premature solidification.
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