A) เคเคฎเคเฅเคฒเคพ เคฎเฅเคเฅเคเคเคพ / เคเคนเคฐเคพ เคเฅเคฒเคพเคฌเฅ (Magenta)
B) เคเคนเคฐเคพ เคชเฅเคฐเคถเคฟเคฏเคจ เคจเฅเคฒเคพ
C) เคธเฅเคฌ เคเฅเคธเคพ เคนเคฐเคพ (Apple green)
D) เคธเฅเคจเคนเคฐเคพ เคญเฅเคฐเคพ
Answer: (A)
The reaction between liberated tissue aldehydes and colorless leucofuchsin (Schiff's reagent) reconstitutes a stable bright magenta-pink quinonoid dye complex.
B) เคนเคพเคฏเคฒเฅเคฐเฅเคจเคฟเคกเฅเค (Hyaluronidase)
C) เค เคฒเฅเคซเคพ-เคเคฎเคพเคเคฒเฅเค / เคกเคพเคฏเคธเฅเคเฅเคธ (Diastase)
D) เคเฅเคฐเคฟเคชเฅเคธเคฟเคจ (Trypsin)
Answer: (C)
Diastase (or salivary alpha-amylase) digests and removes glycogen from tissue sections. Loss of PAS positivity following diastase digestion confirms glycogen.
Ferric ions react with potassium ferrocyanide in the presence of dilute HCl to precipitate insoluble ferric ferrocyanide, classically known as Prussian blue.
C) เคจเฅเคฏเฅเคเฅเคฒเคฟเคฏเคฐ เคซเคพเคธเฅเค เคฐเฅเคก (Nuclear Fast Red)
D) เคฎเฅเคฅเคฟเคฒเฅเคจ เคฌเฅเคฒเฅ
Answer: (C)
Nuclear Fast Red (Kernechtrot) stains cell nuclei pink-red and background cytoplasm pale pink, providing optimal contrast against the deep blue Prussian blue granules.
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