Biolistics (gene gun) is suitable for introducing rDNA into plant cells
Q2. In genetic engineering experiments, restriction enzymes are used for
A) viral DNA
B) bacterial DNA
C) eukaryotic DNA
D) any type of DNA.
Answer: (B) bacterial DNA
In genetic engineering experiments, restriction enzymes are used for bacterial DNA
Q3. The DNA fragments produced by the use of restriction endonucleases can be separated by
A) polymerase chain reaction
B) gel electrophoresis
C) density gradient centrifugation
D) any of the above
Answer: (B) gel electrophoresis
The DNA fragments produced by the use of restriction endonucleases can be separated by gel electrophoresis
Q4. Plasmids in bacterial cells are
A) extra-chromosomal DNA, which cannot replicate
B) extra-chromosomal DNA, which can . self-replicate
C) extra DNA associated with the genome
D) extra DNA, associated with the genome, but cannot replicate.
Answer: ()
Plasmids in bacterial cells are extra-chromosomal DNA, which can . self-replicate
Q5. The DNA polymerase enzyme used in PCR is obtained from
A) Thermus aquaticus
B) Escherichia coli
C) Agrobacterium tumefaciens
D) Salmonella typhimurium
Answer: (A) Thermus aquaticus
The DNA polymerase enzyme used in PCR is obtained from Thermus aquaticus
Q6. While isolating DNA from bacteria, which of the following enzymes is not used?
A) Lysozyme
B) Ribonuclease
C) Deoxyribonuclease
D) Protease
Answer: (A) Lysozyme
Lysozyme is not used
Q7. Significance of ‘heat shock’ method in bacterial transformation is to facilitate
A) binding of DNA to the cell wall
B) uptake of DNA through membrane transport proteins
C) uptake of DNA through transient pores in the bacterial cell wall
D) expression of antibiotic resistance gene
Answer: (C) uptake of DNA through transient pores in the bacterial cell wall
uptake of DNA through transient pores in the bacterial cell wall
Q8. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
A) Denaturation of template DNA
B) Annealing of primers to template DNA
C) Extension of primer end on the template DNA
D) All of the above
Answer: (C) Extension of primer end on the template DNA
Q9. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
A) DNA can be seen in visible light
B) DNA can be seen without staining in visible light
C) Ethidium bromide stained DNA can be seen in visible light
D) Ethidium bromide stained DNA can be seen under exposure to UV light
Answer: (D) Ethidium bromide stained DNA can be seen under exposure to UV light
Ethidium bromide stained DNA can be seen under exposure to UV light is the correct answer
Q10. Restriction’ in Restriction enzyme refers to
A) cleaving ofphosphodiester bond in DNA by the enzyme
B) cutting of DNA at specific position only
C) prevention of the multiplication of bacteriophage in bacteria
D) all of the above
Answer: (C) prevention of the multiplication of bacteriophage in bacteria
Restriction’ in Restriction enzyme refers to prevention of the multiplication of bacteriophage in bacteria
Q11. The role of DNA ligase in the construction of a recombinant DNA molecule is
A) formation of phosphodiester bond between two DNA fragments
B) formation of hydrogen bonds between sticky ends of DNA fragments
C) ligation of all purine and pyrimidine bases
D) none of the above
Answer: (B) formation of hydrogen bonds between sticky ends of DNA fragments
Q12. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
A) both transformants and non-transformants cannot survive
B) both transformants and non-transformants can survive.
C) transformants only and not the non-transformants can survive
D) transformants cannot survive, but non-transformants can not.
Answer: (C) transformants only and not the non-transformants can survive
transformants only and not the non-transformants can survive
Q13. DNA element with ability to change its position is called
A) Cistron
B) Transposon
C) Intron
D) Ricon
Answer: (C) Intron
DNA element with ability to change its position is called Intron
Q14. Viral genome incroporatcd into host DNA is called:
A) Prophase
B) Protophage
C) Bacteriophage
D) None of these
Answer: (C) Bacteriophage
Viral genome incroporatcd into host DNA is called Bacteriophage
Q15. Triticale, first man made cereal crop, has been obtained by crossing wheat with:
A) Rye
B) Pearl millet
C) Sugarcane
D) Barley
Answer: (D) Barley
Triticale, first man made cereal crop, has been obtained by crossing wheat with Barley
Q16. Golden rice is a promising transgenic crop. When released for cultivation, it will help in :
A) High lysin content
B) Pest resistance
C) High protien content
D) High vitamin A content
Answer: (D) High vitamin A content
It will help in High vitamin A content.
Q17. Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from
A) Plasmids
B) AH prokaryotic cells
C) Bacteriophages
D) Bacterial cells
Answer: (D) Bacterial cells
Restrictipn endonucleases are most widely used in recombinant DNA technology. They are obtained from Bacterial cells
Q18. Restriction endonuclease cuts
A) One stand of DNA at specific site
B) Both strands of DNA
C) Both strands of DNA at any site
D) Single strand of RNA
Answer: (B) Both strands of DNA
Restriction endonuclease cuts both strands of DNA
Q19. Plasmid is a
A) Fungus
B) Plasmid
C) Part of Plasma membrane
D) Extra chromosomal DNA in bacterial cell
Answer: (D) Extra chromosomal DNA in bacterial cell
Plasmid is a extra chromosomal DNA in bacterial cell
Q20. Two microbes found tb be very useful in Genetic Engineering are
A) Excherichia coli and Agrobacterium tumefaciens
B) Vibrio cholarae and a tailed bacteriophage
C) Diplococcus sp. and Pseudomonas sp.
D) Crown gall bacterium and Caenorhabdities elegans
Answer: (A) Excherichia coli and Agrobacterium tumefaciens
Excherichia coli and Agrobacterium tumefaciens are two microbes found to be very useful in Genetic Engineering
Q21. DNA obtained for DNA finger printing:
A) W.B.C
B) Hair root cells
C) Body fluids
D) None of these
Answer: (A) W.B.C
Q22. Who discovered Restricted endonucleas enzymes?
A) Smith and Nathane
B) Baiger
C) Waxman
D) Fleming
Answer: (A) Smith and Nathane
Smith and Nathane discovered Restricted endonucleas enzymes
Q23. Two microbes found to be very useful in genetic, engineering are
A) Escherichiya Coli and Agrobacterium tumefaciens
B) Virbio chloerae and tailed bacteriophage
C) Diplococcus sp and pseudomonas sp
D) None of these
Answer: (A) Escherichiya Coli and Agrobacterium tumefaciens
Two microbes found to be very useful in genetic, engineering are Escherichiya Coli and Agrobacterium tumefaciens
Q24. Transgenic plants are develop by
A) Introducing of foreign fenes
B) Clone and genetically modified genes
C) Genetic engineering
D) Purified genes
Answer: (A) Introducing of foreign fenes
Transgenic plants are develop by Introducing of foreign genes
Q25. Transgenic plants are develop by
A) Introducing of foreign fenes
B) Clone and genetically modified genes
C) Genetic engineering
D) Purified genes
Answer: (A) Introducing of foreign fenes
Transgenic plants are develop by Introducing of foreign genes
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