The peptide bond in proteins is detected by

a) Biuret test | In the presence of peptides, a copper (ii) ion forms violet coloured complexes in alkali solutions.

Which is the smallest amino acid?

a) Glycine | Glycine is the smallest amino acid with only 'H' as the residue.

Vitamin A is related to:

a) β-ionone | Vitamin A is composed of β -ionone ring (cyclohexenyl) to which polyisoprenoid side chain is attached.

Vitamins that bind to Ca++ ATPase in intestinal cells is:

d) Vitamin D | Vitamin D is fat soluble responsible for enhancing intestinal absoption of calcium, Iron, Magnesium, Phosphate and zinc.

Regular use of isoniazid as antitubercular drug causes deficiency of:

b) vitamin B6 | Isoniazid causes depletion of vitamin B6 (Pyridoxin).

Sakaguchi's test is specified to:

b) Arginine | Sakaguch's test is used to detect the Arginine in proteins. The guanidine group in arginine reacts with sakaguch's reagent to form red coloured complex.

Which Enzyme is useful for the clinical diagnosis of acute pancreatitis?

d) Both A and B | Acute pancreatitis is sudden swelling and inflammation of the pancreas. It is due to the billiary tract disease, heavy alcohol, idiopathic causes.

Which enzyme catalyse the linking of two compounds?

c) Ligase | Ligase is an enzyme that can catalyse the joining of two large molecules by forming a chemical bond.

Enzyme Reaction kinetics are governed by:

c) Michaellis and Menten theory | Mechellis and Menten theory is one of the best models of enzyme kinetics. The models take the form of an equation describing the rate of enzymatic reaction , relating reaction rate, concentration of the substrate.

What is the source of carbon atoms in Cholesterol?

a) Acetyl-CoA | Acetyl-CoA is an important molecule in metabolism used in many biochemical reactions.

The Enzymes of HMP pathway are located in:

a) Cytosol | Cytosol is the liquid found inside cells. It is seprated into compartment by membranes. For example Mitochondrial matrix, seprates the mitochondrion into many compartments.

The citrate is converted into isocitrate via cis-aconitase by the enzyme:

b) aconitase | Aconitase is an enzyme that catalyse the sterio-specific isomerisation of citrate to isocitrate in the tricarboxylic acid cycle

The disease albinism is related to:

c) defect related to melanin biosynthesis | Albinism is the rare group of genetic desorder that causes the skin, hair, eyes to have little or no colour.

The normal level of calcium in blood plasma is:

c) 9-11 mg/100 ml | the calcium is present plasma of blood in concentration of 9 to 11mg per 100ml

Barfoed's reagent is a solution of:

b) copper acetate in acetic acid | Barfoed's reagent is used for detection of monosachrides in carbohydrate, based on the reaction of copper acetate to copper oxide. RCH + 2Cu2+ + 2 H2O &rarr; RCOOH + 2Cu2O + NH+

The risk factor for atherosclerosis is related to:

d) VLDL and LDL | LDL diposits excess cholesterol in the walls of blood vessels, so it is also called bad cholesterol.

The precursor for Vitamin D is:

c) Cholesterol | Several forms of vitamin D exists. The two major forms are vitamin D2 or ergocalciferol and vitamin D3 or Cholecalciferol.

b) Tripeptide | Glutathione is an important anti-oxidant in plants, animals, fungi and some bacteria. It is a tripeptide with a gamma peptide linkage between the carboxyl groups.

Bial's Reagent is a solution of:

d) Orcinol in 30% HCL + ferric chloride | Bial's test is achemical test for the presence of pentose. It contains 0.4 g of orcinol, 200ml of conc. HCL and 0.5 m of 10% solution of ferric chloride.

Fluorosis is caused by the excess intake of:

d) Fluorine | It is developemental disturbance in enamel caused by excessive exposure to high concentration of fluorine intake.

c) change in optical rotation | Mutarotation is the change in the optical rotation that occurs by epimerization. The optical rotation of the solution depends on the optical rotation of each anomer and their ratio in the solution.

Knoop's beta oxidation is related to:

Q: Vitamin A is related to:

a) &beta;-ionone | Vitamin A is composed of β -ionone ring (cyclohexenyl) to which polyisoprenoid side chain is attached.

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Q.16. Knoop's beta oxidation is related to:

oxidation of fatty acids
oxidation of acetyl Coenzyme
oxidation of keto-bodies
oxidation of uric acid

Answer: (a) oxidation of fatty acids

Biochemistry Practice Test 1 (For Pharmacy, Paramedical And Other Entrance Exams).

25 Questions 482 Attempts 20.27K Seen

Biochemistry is the most important part of medical science which deals with the study of what makes the living system (i.e proteins, DNA, RNA) and what required for living systems (i.e. carbohydrates, minerals, vitamins, enzymes).

Attempt this test to get a basic understanding of what you have learned in Biochemistry. This Biochemistry practice test contains Important MCQs from Biochemistry according to the syllabus of Diploma in Pharmacy. This Test will also helpful for you if you are preparing for other competitive exams like DUET, JNU, BHU, etc. After attempting the test. you can view the complete synopsis of your score, answers, and explanation for each answer. This will help you to increase your preparation strategy for upcoming competitive exams.

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Q.No.	Que title	Option 1 (a)	Option 2 (b)	Option 3 (c)	Option 4 (d)
1	The peptide bond in proteins is detected by	Biuret test	formaldehyde	Million test	water
2	Which is the smallest amino acid?	Glycine	Alanine	Valine	Phenylalanine
3	Vitamin A is related to:	β-ionone	α-ionone	δ-ionone	ɣ-ionone
4	Vitamins that bind to Ca++ ATPase in intestinal cells is:	Vitamin B1	Vitamin B2	Vitamin B6	Vitamin D
5	Regular use of isoniazid as antitubercular drug causes deficiency of:	Vitamin B2	vitamin B6	Vitamin B12	Vitamin B1
6	Sakaguchi's test is specified to:	Glycine	Arginine	Tryptophan	Tyrosine
7	Which Enzyme is useful for the clinical diagnosis of acute pancreatitis?	Lipase	Amylase	Creatinine phosphokinase	Both A and B
8	Which enzyme catalyse the linking of two compounds?	Transferase	Isomerase	Ligase	Lyases
9	Enzyme Reaction kinetics are governed by:	Michaelis theory	Menten theory	Michaellis and Menten theory	Noyes and Whitney theory
10	What is the source of carbon atoms in Cholesterol?	Acetyl-CoA	Lanosterol	Squalene	Mevalonic acid
11	The Enzymes of HMP pathway are located in:	Cytosol	Adipose tissue	Heart	Intestine
12	The citrate is converted into isocitrate via cis-aconitase by the enzyme:	Cutrate synthase	aconitase	isocitrate dehydrogenate	malate dehydrogenase
13	In Glycolysis under anaerobic condition, there is a net gain of:	4 ATP	3 ATP	2 ATP	1 ATP
14	Which is the major pathway/cycle for removal of amonia in brain?	Glutamine pathway	Kreb's Henseleit cycle	Urea synthesis pathway	None of these
15	The disease albinism is related to:	defect of tyrosine metabolism	defect of phenylealanine metabolism	defect related to melanin biosynthesis	All of these
16	Knoop's beta oxidation is related to:	oxidation of fatty acids	oxidation of acetyl Coenzyme	oxidation of keto-bodies	oxidation of uric acid
17	The normal level of calcium in blood plasma is:	20-40 mg/ 100ml	30-40 mg/100 ml	9-11 mg/100 ml	100mg/100 ml
18	Xanthine oxidase contains:	Zinc	Molybdenum	cobalt	magnesium
19	Barfoed's reagent is a solution of:	copper sulphate	copper acetate in acetic acid	cupric tartrate	cupric nitrate
20	The risk factor for atherosclerosis is related to:	Chylomicron	VLDL	HDL	VLDL and LDL
21	The precursor for Vitamin D is:	Fatty acid	Sterol	Cholesterol	Lipid
22	Glutathione is:	Dipeptide	Tripeptide	Cyclic peptide	Polypeptide
23	Bial's Reagent is a solution of:	Alpha napthol in alcohol	Phenol in 30% HCL	Orcinol in 30% HCL	Orcinol in 30% HCL + ferric chloride
24	Fluorosis is caused by the excess intake of:	Copper	Calcium	Zinc	Fluorine
25	Mutarotation is:	change in structure	change in cyclic structure	change in optical rotation	none of above

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